Peiyuan-Huayu Formula Suppresses Proliferation Migration and Angiogenesis of Human Microvascular Endothelial Cells-1 Through Activating MEK/ERK Signaling Pathway

Chronic subdural hematoma is identified as an intracranial hemorrhagic disorder of human. Presently, neither surgical nor symptomatic treatment Western medicine has achieved good curative effects. This study aimed to clarify efficacy Peiyuan-Huayu formula on neovascularization and explore associated mechanisms. Rats were intragastricly administrated with drug containing serum was harvested. Cell counting kit 8 assay used determine proliferation human microvascular endothelial-1 cells. Human cells divided into negative group, blank-serum group drug-serum group. In vitro scratch healing employed evaluate migration distance endothelial cells-1. Tube-like structures formation based conducted using tube matrigel. Expression vascular growth factor A, mitogen-activated protein kinase kinase, phosphorylation extracellular signal-regulated determined western blotting. The 15 % optimal concentration for inhibiting cells-1 proliferation. remarkably inhibited compared Blank-serum (p<0.05). obviously suppressed angiogenesis down-regulated A expression, activated mitogenactivated induced 1/2 in conclusion, proliferation, Effects mediated by reducing expression 1/2, molecule.